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pcr-sscpの例文

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  1. Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k . a 109bp fragment was amplified from the human genome dna . 2 . in 166 han samples , 19 ( 11 . 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
  2. This research began with detecting the rpob gene mutations of 37 mycobacterium tuberculosis clinical isolates by polymerase chain reaction - single strand conformation polymorphism ( pcr - sscp ) to study the application value of this method as a new molecule testing method for drug susceptibility
  3. By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ) , the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated . 3 . the molecular beacon ( mb ) hybridization technique with visual monitoring was established
  4. Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

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