- Traditional affinity columns are used as a preparative step to flush out unwanted biomolecules.
- May be completed using DNA isolation kits available commercially which are based on affinity columns.
- After the protein of interest has been washed through two affinity columns, it can be examined for binding partners.
- Whereas gel agglutination is based on size exclusion of agglutinated red cells in an inert matrix, red cell affinity column technology ( ReACT ) is based on affinity adherence of red cell s in an immunologically active matrix.
- The dissociation constant of the S-peptide for the S-protein is roughly 30 pM; this tight binding can be exploited for protein purification by attaching the S-peptide to the protein of interest and passing a mixture over an affinity column with bound S-protein . [ A smaller C-peptide ( residues 1-13 ) also works . ] The RNase S model system has also been used for studying protein folding by coupling folding and association.