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- These double-strand breaks can be caused by radiation, as well as chemical mutagens.
- Many chemical mutagens require "'biological activation "'to become mutagenic.
- "Mardi also uses induced mutation with physical and chemical mutagens as we did for pisang rastali and pisang berangan, " says Siti Hawa.
- The starting gene can be mutagenised by random point mutations ( by chemical mutagens or error prone PCR ) and proportion of functional variants it contains.
- Initially, the ability of radiation and chemical mutagens to cause mutation was exploited to generate random mutations, but later techniques were developed to introduce specific mutations.