pcr cloningの例文

例文

1. This is particularly dramatic considering that previous studies employing more traditional PCR cloning techniques have resulted in estimates of up to 500 ( Pedr髎-Al韔, 2007 ).
2. There are several advantages that make SB a highly attractive mutagen geared toward gene discovery : 1 ) it has little bias for inserting within particular genomic regions or within specific recognition sequences, 2 ) de novo insertions of the transposon provide a  tagged sequence marker for rapid identification of the specific mutation by simple PCR cloning methods, 3 ) in vivo SB insertional mutagenesis allows multiple mutations to be quickly and easily generated in a single animal, and in a single tissue, such as an adenomatous polyp.