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pcr-sscpの例文

例文モバイル版
  • Pcr - sscp analysis on exon 1 of myogenin gene in sheep
  • The application of pcr - sscp in forensic mtdna typing
  • Polymorphism of brucella omp28 analyzed by pcr - sscp
  • Pcr - sscp analysis on partial sequence of gonadotropin releasing hormone receptor gene in sheep
  • Methods dna sequences of the coagulase were examined by polymerase chain reaction - sigle strand conformation polymorphism ( pcr - sscp ) method and were identified by sequencing
  • But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation
  • With strong specificity and easy to manipulation , this simple , inexpensive , rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp , which shows better advantage than pcr - sscp . 2
  • The research results further prove that resistance to rifampin in mycobacterium tuberculosis resulted from the rpob gene mutation and pcr - sscp can quickly detect rifampin resistance in mycobacterium tuberculosis clinical isolates
  • Compared with the results of dma direct sequencing , pcr - sscp has the same results as that except for failing to check out the rpob gene mutation of a rifampin - resistant isolate . it proves that pcr - sscp is a rapid and sensitive mutation testing method
  • To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid , the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis , and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination
  • Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k . a 109bp fragment was amplified from the human genome dna . 2 . in 166 han samples , 19 ( 11 . 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
  • This research began with detecting the rpob gene mutations of 37 mycobacterium tuberculosis clinical isolates by polymerase chain reaction - single strand conformation polymorphism ( pcr - sscp ) to study the application value of this method as a new molecule testing method for drug susceptibility
  • By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ) , the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated . 3 . the molecular beacon ( mb ) hybridization technique with visual monitoring was established
  • Methods : 1 ) 12h after irradiation , the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp . 2 ) the content of mda , the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation . 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation